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Biochem Biophys Res Commun. 2009 Oct 16;388(2):406-12. doi: 10.1016/j.bbrc.2009.08.022. Epub 2009 Aug 8.

Pulsatile shear stress increased mitochondrial membrane potential: implication of Mn-SOD.

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  • 1Department of Biomedical Engineering and Division of Cardiovascular Medicine, University of Southern California, Los Angeles, CA, USA.

Abstract

Mitochondrial dysfunction is intimately involved in cardiovascular diseases. Mitochondrial membrane potential (DeltaPsi(m)) is coupled with oxidative phosphorylation to drive ATP synthesis. In this study, we examined the effect of physiological pulsatile shear stress (PSS) on DeltaPsi(m) and the role of Mn-SOD expression on DeltaPsi(m). Confluent human aortic endothelial cells (HAEC) were exposed to PSS, and DeltaPsi(m) was monitored using tetramethylrhodamine methyl ester (TMRM(+)), a mitochondrial membrane potential probe. PSS significantly increased DeltaPsi(m) and the change in DeltaPsi(m) was a dynamic process. DeltaPsi(m) returned to baseline level after PSS for 2h followed by static state for 4h. Mitochondrial Mn-SOD expression and activities were also significantly up-regulated in response to PSS. Silencing Mn-SOD attenuated PSS-mediated DeltaPsi(m) increase while adding Mn-SOD mimetic, MnTMPyP, increased DeltaPsi(m) to the similar extent as induced by PSS. Our findings suggest that PSS-increased mitochondrial DeltaPsi(m), in part, via Mn-SOD up-regulation.

PMID:
19666009
[PubMed - indexed for MEDLINE]
PMCID:
PMC2746082
Free PMC Article
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