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Cell. 2009 Aug 21;138(4):795-806. doi: 10.1016/j.cell.2009.05.051. Epub 2009 Aug 6.

Full dynamic range proteome analysis of S. cerevisiae by targeted proteomics.

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  • 1Institute of Molecular Systems Biology, ETH Zurich, Zurich CH 8093, Switzerland.

Abstract

The rise of systems biology implied a growing demand for highly sensitive techniques for the fast and consistent detection and quantification of target sets of proteins across multiple samples. This is only partly achieved by classical mass spectrometry or affinity-based methods. We applied a targeted proteomics approach based on selected reaction monitoring (SRM) to detect and quantify proteins expressed to a concentration below 50 copies/cell in total S. cerevisiae digests. The detection range can be extended to single-digit copies/cell and to proteins undetected by classical methods. We illustrate the power of the technique by the consistent and fast measurement of a network of proteins spanning the entire abundance range over a growth time course of S. cerevisiae transiting through a series of metabolic phases. We therefore demonstrate the potential of SRM-based proteomics to provide assays for the measurement of any set of proteins of interest in yeast at high-throughput and quantitative accuracy.

PMID:
19664813
[PubMed - indexed for MEDLINE]
PMCID:
PMC2825542
Free PMC Article

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