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Ann Otol Rhinol Laryngol. 2009 Jun;118(6):465-8.

New mouse model for studying laryngeal transplantation.

Author information

  • 1Head and Neck Institute, Cleveland Clinic, Cleveland, Ohio, USA. tshipchandler@hotmail.com

Abstract

OBJECTIVES:

Laryngeal transplantation research has been studied in various animal models. For in-depth, immunology-based transplantation research, however, a thoroughly studied animal model must exist. The purpose of this study was to develop a reliable surgical technique in mice to serve as a model for further study of laryngeal transplantation.

METHODS:

Heterotopic laryngeal transplantation was attempted in 15 immunocompetent mice by use of modifications of previously described techniques established in rats.

RESULTS:

Various microvascular techniques were used that led to 8 successful transplants (of 15) with patent vascularity at the time of sacrifice. The first 7 attempts at transplantation were unsuccessful because of technical difficulties related to vessel size, soft tissue traumatic injury, and venous congestion. Subsequently, 8 transplantation procedures were successfully performed after modifications of the surgical technique.

CONCLUSIONS:

This pilot study describes the reproducible surgical techniques performed in using mice for studying laryngeal transplantation. Mice are cost-effective and immunologically well studied, and are thus ideal for further laryngeal transplantation research.

PMID:
19663379
[PubMed - indexed for MEDLINE]
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