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J Immunol Methods. 2009 Sep 30;349(1-2):56-60. doi: 10.1016/j.jim.2009.07.013. Epub 2009 Aug 3.

A method for isolating and analyzing human mRNA from newborn stool.

Author information

  • 1Washington University School of Medicine, Department of Pediatrics, Division of Pediatric Gastroenterology, Washington, United States of America.

Abstract

Efforts to characterize the human transcriptome have largely been limited to blood, urine, and tissue analyses (i.e., normally sterile materials). We report here an extraction protocol using commercially available reagents to obtain high-yield, reverse-transcribable RNA from human stool. Quantitative reverse transcriptase polymerase chain reactions demonstrated minimal intra-specimen but considerable intra-subject variability over time of transcripts for interleukin-6 (IL-6), IL-8, epidermal growth factor (EGF), calprotectin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). This technique now expands opportunities to use the human fecal transcriptome to characterize gastrointestinal pathophysiology.

PMID:
19660464
[PubMed - indexed for MEDLINE]
PMCID:
PMC2850193
Free PMC Article

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