Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Opt Express. 2009 Aug 3;17(16):13447-57.

Detecting intrinsic scattering changes correlated to neuron action potentials using optical coherence imaging.

Author information

  • 1Department of Electrical and Computer Engineering, Biophotonics Imaging Laboratory, Beckman Institute for Advanced Science and Technology University of Illinois at Urbana-Champaign405 N. Mathews Avenue, Urbana, IL 61801, USA.

Abstract

We demonstrate how optical coherence imaging techniques can detect intrinsic scattering changes that occur during action potentials in single neurons. Using optical coherence tomography (OCT), an increase in scattering intensity from neurons in the abdominal ganglion of Aplysia californica is observed following electrical stimulation of the connective nerve. In addition, optical coherence microscopy (OCM), with its superior transverse spatial resolution, is used to demonstrate a direct correlation between scattering intensity changes and membrane voltage in single cultured Aplysia bag cell neurons during evoked action potentials. While intrinsic scattering changes are small, OCT and OCM have potential use as tools in neuroscience research for non-invasive and non-contact measurement of neural activity without electrodes or fluorescent dyes. These techniques have many attractive features such as high sensitivity and deep imaging penetration depth, as well as high temporal and spatial resolution. This study demonstrates the first use of OCT and OCM to detect functionally-correlated optical scattering changes in single neurons.

PMID:
19654752
[PubMed - indexed for MEDLINE]
PMCID:
PMC2883318
Free PMC Article

Images from this publication.See all images (6)Free text

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Optical Society of America Icon for PubMed Central
    Loading ...
    Write to the Help Desk