Format

Send to:

Choose Destination
See comment in PubMed Commons below
Opt Express. 2009 Jul 20;17(15):13080-94.

Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells.

Author information

  • 1Aix-Marseille Université, Ecole Centrale Marseille, CNRS, Institut Fresnel, Campus de Saint-Jérôme, 13013 Marseille, France. pierre.bon@fresnel.fr

Abstract

Phase imaging with a high-resolution wavefront sensor is considered. This is based on a quadriwave lateral shearing interferometer mounted on a non-modified transmission white-light microscope. The measurement technology is explained both in the scope of wave optics and geometrical optics in order to discuss its implementation on a conventional microscope. In particular we consider the effect of a non spatially coherent source on the phase-image signal-to-noise ratio. Precise measurements of the phase-shift introduced by microscopic beads or giant unilamellar vesicles validate the principle and show the accuracy of the methods. Diffraction limited images of living COS-7 cells are then presented, with a particular focus on the membrane and organelle dynamics.

PMID:
19654713
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Optical Society of America
    Loading ...
    Write to the Help Desk