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Opt Express. 2009 Jul 20;17(15):13080-94.

Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells.

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  • 1Aix-Marseille Université, Ecole Centrale Marseille, CNRS, Institut Fresnel, Campus de Saint-Jérôme, 13013 Marseille, France.


Phase imaging with a high-resolution wavefront sensor is considered. This is based on a quadriwave lateral shearing interferometer mounted on a non-modified transmission white-light microscope. The measurement technology is explained both in the scope of wave optics and geometrical optics in order to discuss its implementation on a conventional microscope. In particular we consider the effect of a non spatially coherent source on the phase-image signal-to-noise ratio. Precise measurements of the phase-shift introduced by microscopic beads or giant unilamellar vesicles validate the principle and show the accuracy of the methods. Diffraction limited images of living COS-7 cells are then presented, with a particular focus on the membrane and organelle dynamics.

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