Anal Biochem. 2009 Dec 1;395(1):116-8. Epub 2009 Aug 3.

Method for the simultaneous quantitation of apolipoprotein E isoforms using tandem mass spectrometry.

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Department of Neurology, Hope Center for Neurological Disorders and Alzheimer's Disease Research Center, Washington University School of Medicine, St. Louis, MO 63110, USA.

Abstract

Using apolipoprotein E (ApoE) as a model protein, we developed a protein isoform analysis method utilizing stable isotope labeling tandem mass spectrometry (SILT MS). ApoE isoforms are quantitated using the intensities of the b and y ions of the (13)C-labeled tryptic isoform-specific peptides versus unlabeled tryptic isoform-specific peptides. The ApoE protein isoform analysis using SILT allows for the simultaneous detection and relative quantitation of different ApoE isoforms from the same sample. This method provides a less biased assessment of ApoE isoforms compared to antibody-dependent methods, and may lead to a better understanding of the biological differences between isoforms.

PMID:: 19653990; [PubMed - indexed for MEDLINE]
PMCID: PMC2749897

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