Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Stem Cells. 2009 Nov;27(11):2691-702. doi: 10.1002/stem.183.

Lsh participates in DNA methylation and silencing of stem cell genes.

Author information

  • 1Laboratory of Cancer Prevention, SAIC-Frederick, National Cancer Institute, Frederick, MD 21701, USA.

Abstract

Transcriptional control of stem cell genes is a critical step in differentiation of embryonic stem cells and in reprogramming of somatic cells into stem cells. Here we report that Lsh, a regulator of repressive chromatin at retrotransposons, also plays an important role in silencing of stem cell-specific genes such as Oct4. We found that CpG methylation is gained during in vitro differentiation of several stem cell-specific genes (in 11 of 12 promoter regions) and thus appears to be a common epigenetic mark. Lsh depletion prevents complete silencing of stem cell gene expression and moreover promotes the maintenance of stem cell characteristics in culture. Lsh is required for establishment of DNA methylation patterns at stem cell genes during differentiation, in part by regulating access of Dnmt3b to its genomic targets. Our results indicate that Lsh is involved in the control of stem cell genes and suggest that Lsh is an important epigenetic modulator during early stem cell differentiation.

PMID:
19650037
[PubMed - indexed for MEDLINE]
PMCID:
PMC3327128
Free PMC Article

Images from this publication.See all images (7)Free text

Fig 1
Fig 2
Fig 3
Fig 4
Fig 5
Fig 6
Fig 7
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for John Wiley & Sons, Inc. Icon for PubMed Central
    Loading ...
    Write to the Help Desk