Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
IEEE Trans Med Imaging. 2010 Jan;29(1):96-105. doi: 10.1109/TMI.2009.2027813. Epub 2009 Jul 28.

Multiple nuclei tracking using integer programming for quantitative cancer cell cycle analysis.

Author information

  • 1Department of Information Science, School of Mathematical Sciences, and LMAM, Peking University, Beijing 100871, China. robert.fh.li@gmail.com

Abstract

Automated cell segmentation and tracking are critical for quantitative analysis of cell cycle behavior using time-lapse fluorescence microscopy. However, the complex, dynamic cell cycle behavior poses new challenges to the existing image segmentation and tracking methods. This paper presents a fully automated tracking method for quantitative cell cycle analysis. In the proposed tracking method, we introduce a neighboring graph to characterize the spatial distribution of neighboring nuclei, and a novel dissimilarity measure is designed based on the spatial distribution, nuclei morphological appearance, migration, and intensity information. Then, we employ the integer programming and division matching strategy, together with the novel dissimilarity measure, to track cell nuclei. We applied this new tracking method for the tracking of HeLa cancer cells over several cell cycles, and the validation results showed that the high accuracy for segmentation and tracking at 99.5% and 90.0%, respectively. The tracking method has been implemented in the cell-cycle analysis software package, DCELLIQ, which is freely available.

PMID:
19643704
[PubMed - indexed for MEDLINE]
PMCID:
PMC2846554
Free PMC Article

Images from this publication.See all images (14)Free text

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12
Fig. 13
Fig. 14
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for IEEE Engineering in Medicine and Biology Society Icon for PubMed Central
    Loading ...
    Write to the Help Desk