Quantification of the Oregon Green 488-labeled pDNA nuclear fluorescence intensity in the central confocal sections is shown. Bars represent mean ± S.E. of the mean background-subtracted Oregon Green/pCMVβ-DTS nuclei fluorescence intensity (n = 33–42 nuclei). The asterisk indicates significant (p < 0.001) difference from import activity supported by recombinant importin-α, -β, and Ran alone.

Plasmids (A). Abbreviated elements are as follows: CMViep, CMV immediate early promoter/enhancer; Chx10 or NM23-H2, binding sequences from shuttle proteins, Chx10 and NM23-H2, respectively; SV40 DTS, SV40 DNA nuclear targeting sequence. β-Galactosidase reporter gene activity of pCMVβ-DTS/NM23-H2 and pCMVβ-DTS/Chx10 (B). Forty-eight hours after Lipofectamine 2000-mediated transfection of G₁/S-arrested HeLa cells with 1 μg of either control pCMVβ-DTS, pCMVβ-DTS/NM23-H2, or pCMVβ-DTS/Chx10, β-galactosidase activity was assayed. Data represent the mean ratio of the β-galactosidase activity (RLU/mg protein) in cells transfected with pCMVβ-DTS/NM23-H2 or pCMVβ-DTS/Chx10 and pCMVβ-DTS (means ± S.E. error bars; n = 12 dishes from three independent experiments).

Nuclear entry and plasmid DNA nuclear import activity of recombinant NM23-H2. Digitonin-permeabilized HeLa cells were incubated with Oregon Green/pCMVβ-DTS, cytoplasmic extract, Cy5-Hsc70, and Alexa-NM23-H2 in the absence or presence of WGA. After incubation, cells were processed, as indicated under “Experimental Procedures,” and fluorescence images of 0.5-μm optical sections and corresponding phase contrast images visualized using a CLSM. Representative central section fluorescent images matched and superimposed with their respective phase contrast images are shown. Alexa-NM23-H2 in the absence (A–D) or presence (E–H) of WGA (100 μg/ml). Alexa Fluor-555 (red) channel; (A, E), Oregon Green 488 (green) channel; (B, F), Cy-5 (blue) channel; (C, G). Alexa Fluor 555 confocal images for NM23-H2 were matched to their respective Oregon Green-pDNA images and a picture overlay was performed to show co-localization in the absence (D) or presence of WGA (H). Bar, 10 μm. I, quantification of the Alexa-H2B and Hsc70-mediated Alexa-NM23-H2 nuclear fluorescence intensity in the central confocal sections. Bars represent mean ± S.E. of the background-subtracted Oregon Green/pCMVβ-DTS nuclei fluorescence intensity (n = 33–42 nuclei). The asterisk indicates significant (p < 0.001) difference from import activity supported by recombinant importin-α, -β, and Ran alone.

Nuclear entry and plasmid DNA nuclear import activity of recombinant histone H2B. Digitonin-permeabilized HeLa cells were incubated with Oregon Green/pCMVβ-DTS (10 μg/ml), cytoplasmic extract (5 mg protein/ml), and Alexa histone H2B in the absence (A–C) or presence (D–F) of WGA (100 μg/ml). After incubation, cells were processed, as indicated under “Experimental Procedures” and fluorescence images of 0.5 μm optical sections and corresponding phase contrast images visualized using a CLSM. Representative central section fluorescent images matched and superimposed with their respective phase contrast images are shown. Alexa Fluor-555 (red) channel; (A, D), Oregon Green 488 (green) channel; (B, E). Alexa Fluor 555 confocal images for histone H2B were matched to their respective Oregon Green-pDNA images, and a picture overlay was performed using Adobe Photoshop to show co-localization in the absence (C) or presence of WGA (F). Bar, 10 μm. G, quantification of the Alexa-histone H2B nuclear fluorescence intensity in the central confocal sections. The bars represent mean ± S.E. of the background subtracted Oregon Green/pCMVβ-DTS nuclei fluorescence intensity (n = 33 nuclei). The asterisk indicates a significant (p < 0.001) difference from import activity supported by recombinant importin-α, -β, and Ran alone.

Plasmid DNA nuclear import into digitonin-permeabilized HeLa cells. Digitonin-permeabilized HeLa cells were incubated in the presence of recombinant importin-α, -β, and Ran, with Oregon Green/pCMVβ-DTS (10 μg DNA/ml) and native cytoplasmic HeLa (0.5 mg protein/ml). A, confocal z-scan consisting of 8 optical sections (0.5-μm step size) superimposed on their corresponding phase contrast images from the top to the bottom of the nuclei to verify fluorescent pDNA nuclear localization (representative of six similar experiments). B, quantification of the nuclear fluorescence intensity in the central sections of images from the assay performed with native (in the absence or presence of WGA) or heat-inactivated extract. The bars represent mean ± S.E. of the background-subtracted nuclei fluorescent intensity (n = 24–35 nuclei). Cytoplasmic extract was heat-inactivated (90 °C, 10 min) before addition. Asterisks indicate a significant difference from native cytoplasmic extract import activity (p < 0.001). C, depletion of putative DNA-binding factors abolishes the plasmid DNA nuclear import activity from cytoplasmic extracts. The pDNA nuclear import activity of depleted HeLa cytoplasmic extract was compared with that of native and control extracts. Digitonin-permeabilized HeLa cells were incubated with the extracts (5 mg protein/ml) containing the Oregon Green/pCMVβ-DTS (10 μg/ml) in the absence (dark bars) or presence (gray bars) of WGA (100 μg/ml). After incubation, cells were processed for visualization by CLSM. Quantification of the nuclear fluorescence intensity in central sections is shown. 17–42 nuclei were analyzed for each experimental condition. The bars represent mean ± S.E. of the background-subtracted nuclei fluorescent intensity compared with native cytoplasmic extract in the absence of WGA (*, p < 0.001).

Elution profiles of HeLa cell cytoplasmic extracts on a pCMVβ-DTS/PNA-Sepharose column. Cytoplasmic extract (0.5 mg of protein in 500 μl) was applied to the pCMVβ-DTS/PNA-Sepharose column (A) or control PNA-Sepharose column (B), washed with three column volumes of column buffer, and elution achieved with a linear gradient of NaCl (0–0.5 m NaCl, per 20 ml buffer; 0.5 min/ml, 4 °C). Alternate elution fractions (2 ml each) were pooled (denoted numbers 2, 4, 6, 8, 10, 12, 14, 16, 18, and 20), dialyzed overnight against import buffer, concentrated using Centricon-5 microconcentration devices and assayed for Oregon Green/pCMVβ-DTS nuclear import activity. Quantification was performed on 8–59 nuclei for each type of treatment. Percent activity represents the mean background-subtracted nuclear fluorescent intensity as a percentage of the native HeLa cytoplasmic extract import activity. The concentration of the linear NaCl gradient is superimposed on the elution profile. C, fractionation of pDNA nuclear import activity from HeLa cell cytoplasmic extracts using the pCMVβ-DTS/PNA-Sepharose column. Details are as for A and B. Activity was determined in the absence (dark bars) or presence (gray bars) of WGA (100 μg/ml). Data points represent the mean nuclear fluorescence intensity ± S.E. (n = 8–59 nuclei).