Proteomics. 2009 Aug;9(15):3939-44.

Accurate quantitation of standard peptides used for quantitative proteomics.

Bordeerat NK, Georgieva NI, Klapper DG, Collins LB, Cross TJ, Borchers CH, Swenberg JA, Boysen G.

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Department of Environmental Sciences and Engineering, The University of North Carolina at Chapel Hill, USA.

Abstract

MS-based proteomics has become an indispensable tool in system biology generating a need for accurate and precise quantitation of peptide standards. The presented method utilizes ultra performance LC-MS/MS (UPLC-MS/MS) to accurately quantify peptide standards at concentrations of 0.1-10 microM. The ability for accurate quantitation of micro-molar concentrations has the advantages that quantitation can be performed routinely with high precision and the high sensitivity of the method minimizes the amounts required.

PMID:: 19637239; [PubMed - indexed for MEDLINE]
PMCID: PMC2766299

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