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Biomol NMR Assign. 2008 Dec;2(2):211-4. doi: 10.1007/s12104-008-9123-7. Epub 2008 Oct 5.

Backbone assignment of the UHM domain of Puf60 free and bound to five ligands.

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  • 1Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstr. 1, Heidelberg, D-69117, Germany.


U2AF homology motifs (UHM) are protein domains that bind peptidic UHM ligand motifs (ULM) and thus form an intricate network of interactions involved in splicing regulation. Here, we report the backbone assignment of the UHM domain of the splicing factor Puf60 as well as (1)H, (15)N chemical shifts upon binding of the ULM peptides U2AF(65) (85-112), SF1 (1-25), SF3b155 (194-229), SF3b155 (317-357), and Prp16 (201-238).

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