Backbone and sidechain (1)H, (15)N and (13)C assignments of the human G-actin binding protein profilin IIa

Biomol NMR Assign. 2007 Dec;1(2):205-7. doi: 10.1007/s12104-007-9057-5. Epub 2007 Nov 13.

Abstract

The resonance assignment of the human profilin IIa have been determined, based on triple-resonance experiments using uniformly [(13)C,(15)N]-labeled protein. These assignments facilitate further studies of interactions between profilin IIa and its poly-L: -proline rich ligands.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Actins / chemistry*
  • Amino Acid Sequence
  • Carbon Isotopes / chemistry
  • Humans
  • Magnetic Resonance Spectroscopy / methods*
  • Molecular Weight
  • Nitrogen Isotopes / chemistry
  • Profilins / chemistry*
  • Protein Binding
  • Protein Structure, Tertiary
  • Protons

Substances

  • Actins
  • Carbon Isotopes
  • Nitrogen Isotopes
  • Profilins
  • Protons