Format

Send to:

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2009 Aug;6(8):600-2. doi: 10.1038/nmeth.1349. Epub 2009 Jul 26.

The twin spot generator for differential Drosophila lineage analysis.

Author information

  • 1Commissariat à l'Energie Atomique, Institut de Recherches en Technologies et Sciences pour le Vivant, Centre National de la Recherche Scientifique, Grenoble, France. rgriffin@receptor.med.harvard.edu

Abstract

In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies.

PMID:
19633664
[PubMed - indexed for MEDLINE]
PMCID:
PMC2720837
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Write to the Help Desk