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Adv Drug Deliv Rev. 2009 Sep 30;61(11):908-17. doi: 10.1016/j.addr.2009.07.003. Epub 2009 Jul 21.

"Splicing up" drug discovery. Cell-based expression and screening of genetically-encoded libraries of backbone-cyclized polypeptides.

Author information

  • 1Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90033, USA.

Abstract

The present paper reviews the use of protein splicing for the biosynthesis of backbone cyclic polypeptides. This general method allows the in vivo and in vitro biosynthesis of cyclic polypeptides using recombinant DNA expression techniques. Biosynthetic access to backbone cyclic peptides opens the possibility to generate cell-based combinatorial libraries that can be screened inside living cells for their ability to attenuate or inhibit cellular processes thus providing a new way for finding therapeutic agents.

PMID:
19628015
[PubMed - indexed for MEDLINE]
PMCID:
PMC3329944
Free PMC Article

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