Science. 1991 Dec 6;254(5037):1507-9.

Systemic delivery of recombinant proteins by genetically modified myoblasts.

Source

Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor 48109.

Abstract

The ability to stably deliver recombinant proteins to the systemic circulation would facilitate the treatment of a variety of acquired and inherited diseases. To explore the feasibility of the use of genetically engineered myoblasts as a recombinant protein delivery system, stable transfectants of the murine C2C12 myoblast cell line were produced that synthesize and secrete high levels of human growth hormone (hGH) in vitro. Mice injected with hGH-transfected myoblasts had significant levels of hGH in both muscle and serum that were stable for at least 3 weeks after injection. Histological examination of muscles injected with beta-galactosidase-expressing C2C12 myoblasts demonstrated that many of the injected cells had fused to form multinucleated myotubes. Thus, genetically engineered myoblasts can be used for the stable delivery of recombinant proteins into the circulation.

Comment in

Science. 1991 Dec 6;254(5037):1455-6.

PMID:: 1962212; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

1 P01 DK42718-01/DK/NIDDK NIH HHS/United States

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Medical

Genes and Gene Therapy - MedlinePlus Health Information

Supplemental Content

Save items

Related citations in PubMed

Systemic delivery of human growth hormone by injection of genetically engineered myoblasts. [Science. 1991]
Systemic delivery of human growth hormone by injection of genetically engineered myoblasts.
Dhawan J, Pan LC, Pavlath GK, Travis MA, Lanctot AM, Blau HM. Science. 1991 Dec 6; 254(5037):1509-12.
Systemic delivery of a recombinant protein by genetically modified mesothelial cells reseeded on the parietal peritoneal surface. [Gene Ther. 1995]
Systemic delivery of a recombinant protein by genetically modified mesothelial cells reseeded on the parietal peritoneal surface.
Nagy JA, Shockley TR, Masse EM, Harvey VS, Hoff CM, Jackman RW. Gene Ther. 1995 Aug; 2(6):402-10.
Correction of the growth defect in dwarf mice with nonautologous microencapsulated myoblasts--an alternate approach to somatic gene therapy. [Hum Gene Ther. 1995]
Correction of the growth defect in dwarf mice with nonautologous microencapsulated myoblasts--an alternate approach to somatic gene therapy.
al-Hendy A, Hortelano G, Tannenbaum GS, Chang PL. Hum Gene Ther. 1995 Feb; 6(2):165-75.
Review Microcapsules as bio-organs for somatic gene therapy. [Ann N Y Acad Sci. 1997]
Review Microcapsules as bio-organs for somatic gene therapy.
Chang PL. Ann N Y Acad Sci. 1997 Dec 31; 831:461-73.
Review Myoblasts in pattern formation and gene therapy. [Trends Genet. 1993]
Review Myoblasts in pattern formation and gene therapy.
Blau HM, Dhawan J, Pavlath GK. Trends Genet. 1993 Aug; 9(8):269-74.

See reviews... See all...

Cited by 24 PubMed Central articles

Review Advances in musculoskeletal tissue engineering: moving towards therapy. [Organogenesis. 2010]
Review Advances in musculoskeletal tissue engineering: moving towards therapy.
Rossi CA, Pozzobon M, De Coppi P. Organogenesis. 2010 Jul-Sep; 6(3):167-72.
Preclinical assessment of wt GNE gene plasmid for management of hereditary inclusion body myopathy 2 (HIBM2). [Gene Regul Syst Bio. 2008]
Preclinical assessment of wt GNE gene plasmid for management of hereditary inclusion body myopathy 2 (HIBM2).
Jay C, Nemunaitis G, Nemunaitis J, Senzer N, Hinderlich S, Darvish D, Ogden J, Eager J, Tong A, Maples PB. Gene Regul Syst Bio. 2008 Jun 20; 2:243-52. Epub 2008 Jun 20.
IGF-I increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors. [J Cell Biol. 2005]
IGF-I increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors.
Sacco A, Doyonnas R, LaBarge MA, Hammer MM, Kraft P, Blau HM. J Cell Biol. 2005 Nov 7; 171(3):483-92.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Systemic delivery of recombinant proteins by genetically modified myoblasts.
Systemic delivery of recombinant proteins by genetically modified myoblasts.
Science. 1991 Dec 6 ;254(5037):1507-9.

PubMed
Loss of HBsAg antigen during treatment with entecavir or lamivudine in nucleosid...
Loss of HBsAg antigen during treatment with entecavir or lamivudine in nucleoside-naïve HBeAg-positive patients with chronic hepatitis B.
J Viral Hepat. 2010 Jan ;17(1):16-22. Epub 2009 Jul 19 .

PubMed
Plant immunity: a lesson from pathogenic bacterial effector proteins.
Plant immunity: a lesson from pathogenic bacterial effector proteins.
Cell Microbiol. 2009 Oct ;11(10):1453-61. Epub 2009 Jul 20 .

PubMed
Efficient and rapid identification of Candida albicans allelic status using SNP-...
Efficient and rapid identification of Candida albicans allelic status using SNP-RFLP.
FEMS Yeast Res. 2009 Oct ;9(7):1061-9. Epub 2009 Jun 22 .

PubMed
Induction of stem cell gene expression in adult human fibroblasts without transg...
Induction of stem cell gene expression in adult human fibroblasts without transgenes.
Cloning Stem Cells. 2009 Sep ;11(3):417-26.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: