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Nat Methods. 2009 Aug;6(8):593-5. doi: 10.1038/nmeth.1354. Epub 2009 Jul 20.

Virtual terminator nucleotides for next-generation DNA sequencing.

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  • 1Helicos BioSciences Corporation, Cambridge, Massachusetts, USA.

Abstract

We synthesized reversible terminators with tethered inhibitors for next-generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides, and we used them to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high-quality sequence data without the need for target amplification and thus avoided concomitant biases.

PMID:
19620973
[PubMed - indexed for MEDLINE]
PMCID:
PMC2719685
Free PMC Article

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