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Genetics. 2009 Sep;183(1):131-48. doi: 10.1534/genetics.109.106203. Epub 2009 Jul 13.

Transcription independent insulation at TFIIIC-dependent insulators.

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  • 1Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, California 95064, USA.

Abstract

Chromatin insulators separate active from repressed chromatin domains. In yeast the RNA pol III transcription machinery bound to tRNA genes function with histone acetylases and chromatin remodelers to restrict the spread of heterochromatin. Our results collectively demonstrate that binding of TFIIIC is necessary for insulation but binding of TFIIIB along with TFIIIC likely improves the probability of complex formation at an insulator. Insulation by this transcription factor occurs in the absence of RNA polymerase III or polymerase II but requires specific histone acetylases and chromatin remodelers. This analysis identifies a minimal set of factors required for insulation.

PMID:
19596900
[PubMed - indexed for MEDLINE]
PMCID:
PMC2746139
Free PMC Article
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