The nucleotide and deduced amino acid sequences of porcine liver proline- beta-naphthylamidase. Evidence for the identity with carboxylesterase

M Matsushima; H Inoue; M Ichinose; S Tsukada; K Miki; K Kurokawa; T Takahashi; K Takahashi

doi:10.1016/0014-5793(91)81147-z

The nucleotide and deduced amino acid sequences of porcine liver proline- beta-naphthylamidase. Evidence for the identity with carboxylesterase

FEBS Lett. 1991 Nov 18;293(1-2):37-41. doi: 10.1016/0014-5793(91)81147-z.

Authors

M Matsushima¹, H Inoue, M Ichinose, S Tsukada, K Miki, K Kurokawa, T Takahashi, K Takahashi

Affiliation

¹ Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.

PMID: 1959668
DOI: 10.1016/0014-5793(91)81147-z

Abstract

A cDNA clone for porcine liver proline-beta-naphthylamidase was isolated and sequenced. The deduced amino acid sequence of 567 residues was highly homologous with those of carboxylesterases (EC 3.1.1.1) previously reported for other species. In addition, proline-beta-naphthylamidase purified from porcine liver was shown to have strong activity towards p-nitrophenylacetate, a representative substrate for carboxylesterases. These results suggest that proline-beta-naphthylamidase is identical with carboxylesterase.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Aminopeptidases / chemistry
Aminopeptidases / genetics*
Aminopeptidases / isolation & purification
Animals
Base Sequence
Carboxylesterase
Carboxylic Ester Hydrolases / genetics*
DNA / isolation & purification
Microsomes, Liver / enzymology*
Molecular Sequence Data
Rabbits
Rats
Sequence Homology, Nucleic Acid*
Substrate Specificity
Swine

Substances

DNA
Carboxylic Ester Hydrolases
Carboxylesterase
Aminopeptidases

Associated data

GENBANK/S62644
GENBANK/S62648
GENBANK/S62652
GENBANK/S66780
GENBANK/X58803
GENBANK/X63322
GENBANK/X63323
GENBANK/X63567
GENBANK/X63568
GENBANK/X63569