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Appl Opt. 2009 Jul 10;48(20):3998-4002.

Optimization of axial resolution in a confocal microscope with D-shaped apertures.

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  • 1Division of Bioengineering, National University of Singapore, Singapore 117576.


We show theoretically that the axial resolution is improved when two centrosymmetric D-shaped apertures are combined in a confocal microscope with a finite-sized pinhole. The optimum width of a divider that separates the D-shaped apertures to give the maximum axial resolution for a given pinhole size is investigated, and the magnitude of the signal level is explored.

[PubMed - indexed for MEDLINE]
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