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    Biosci Biotechnol Biochem. 2009 Jul;73(7):1661-4. Epub 2009 Jul 7.

    Construction of a protein library of Arabidopsis transcription factors using a wheat cell-free protein production system and its application for DNA binding analysis.

    Nozawa A, Matsubara Y, Tanaka Y, Takahashi H, Akagi T, Seki M, Shinozaki K, Endo Y, Sawasaki T.

    Source

    Cell-Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University, Matsuyama, Ehime, Japan.

    Abstract

    We created a protein library consisting of 647 Arabidopsis transcription factors (TFs) using a wheat cell-free system. The quality of proteins in the library was checked by binding assay of bZIP family proteins. Screening of TFs binding to 5'-regulatory regions of FLC and LFY was conducted using the library, and MYB67 and GBF1 were found to be binding factors.

    PMID:
    19584551
    [PubMed - indexed for MEDLINE]
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