Opt Express. 2008 Nov 10;16(23):18764-9.

Focal modulation microscopy.

Source

Division of Bioengineering, National University of Singapore, Singapore. biecng@nus.edu.sg

Abstract

We report a novel light microscopy method for high resolution molecular imaging of thick biological tissues with one photon excited fluorescence. Effective optical sectioning and diffraction limited spatial resolution are achieved when imaging deep inside a multiple-scattering medium by the use of focal modulation, a technique for suppressing the background fluorescence signal excited by scattered light. Our method has been validated with animal tissue and an imaging depth around 600 microns has been demonstrated.

PMID:: 19581963; [PubMed - indexed for MEDLINE]

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Research Support, Non-U.S. Gov't

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