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    J Sep Sci. 2009 Aug;32(15-16):2691-8.

    Accurate molecular weight analysis of histones using FFE and RP-HPLC on monolithic capillary columns.

    Sneekes EJ, Han J, Elliot M, Ausio J, Swart R, Heck AJ, Borchers C.

    Source

    Dionex Corporation, Amsterdam, The Netherlands.

    Abstract

    Due to their large diversity with respect to post-translational modifications (PTMs), the family of histones provides a major analytical challenge in current proteomics research. Their function has a large impact on the transcription of DNA, and as a result, on the expression of proteins. The variation in PTMs regulates transcription, and, as a result, many methods are being employed for the in-depth analysis of histones. In this paper, we present a separation strategy for histones based on free-flow electrophoresis (FFE) followed by an RP separation on capillary monolithic PS-DVB columns. The capillary columns are directly interfaced with an FT-ICR MS providing an online system for the detection and accurate molecular weight analysis of intact histones.

    PMID:
    19569095
    [PubMed - indexed for MEDLINE]

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