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Proc Natl Acad Sci U S A. 2009 Jul 7;106(27):11090-5. Epub 2009 Jun 22.

Structure and assembly of immature HIV.

Briggs JA, Riches JD, Glass B, Bartonova V, Zanetti G, Kräusslich HG.

Source

Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany. briggs@embl.de

Abstract

The major structural components of HIV are synthesized as a 55-kDa polyprotein, Gag. Particle formation is driven by the self-assembly of Gag into a curved hexameric lattice, the structure of which is poorly understood. We used cryoelectron tomography and contrast-transfer-function corrected subtomogram averaging to study the structure of the assembled immature Gag lattice to approximately 17-A resolution. Gag is arranged in the immature virus as a single, continuous, but incomplete hexameric lattice whose curvature is mediated without a requirement for pentameric defects. The resolution of the structure allows positioning of individual protein domains. High-resolution crystal structures were fitted into the reconstruction to locate protein-protein interfaces involved in Gag assembly, and to identify the structural transformations associated with virus maturation. The results of this study suggest a concept for the formation of nonsymmetrical enveloped viruses of variable sizes.

PMID:: 19549863; [PubMed - indexed for MEDLINE]
PMCID: PMC2700151

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Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

gag Gene Products, Human Immunodeficiency Virus

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