Comparative analysis of the C. difficile 630 spore proteome to 11 clostridial genomes. Diagrams summarize the C. difficile spore protein functional classes shared among clostridia (top), shared between C. difficile and C. bartlettii (center), and unique to C. difficile (bottom).

Representation of the distribution and abundance, by functional class, of C. difficile 630 spore proteins. Shown are the absolute number of proteins (filled bars, left axis) and the percentage of each functional class in spores relative to the total genome (shaded bars, right axis). Functional classes are described in reference 34.

Summary of scheme to determine the spore proteome. (a) SDS-PAGE gel of vegetative cells (veg) (left lane) and pure spores (right lane) after solubilization with LDS. (b) Flow diagram outlining the steps required to solubilize proteins from C. difficile spores and the number of extra proteins liberated at each stage. (c) Rarefaction curve showing the decrease in the number of extra proteins with each additional solubilization step.

Visualization of pure C. difficile spores. (a) Endospore stain of C. difficile culture showing the pink vegetative cells (veg) and pink extracellular matrix (ex), with a few interspersed green spores (sp). (b) Purified C. difficile spores are stained green. (c) Transmission electron microscopy of sectioned C. difficile spores, demonstrating the spore ultrastructure including the exosporium, coat, cortex, core, membrane, and ribosomes. Bar, 100 nm. (d) Magnified section of outer surface of spore. Bar, 50 nm.

Environmental C. difficile 630 spores are highly infective. (a) Infective dose curves of pure and feces-derived C. difficile 630 spores in mice (n = 3) after exposure to environmental spore contamination. Based on the Reed-Muench formulation, the ID₅₀ of environmental spores with a 1-h exposure is 4 spores/cm² for fecal spores (gray lines) and 7 spores/cm² for pure spores (black lines). Data are representative of two independent experiments. (b) Fecal shedding of C. difficile 630 from infected mice (n = 6) demonstrates that spores establish intestinal carriage in infected mice. The broken gray line represents the expected shedding pattern during the induction of high-level C. difficile shedding. The detection limit of 5 × 10¹ C. difficile CFU/g of feces is indicated by a dashed horizontal line.

Pure C. difficile spores are viable and maintain their resistant nature. (a) Germination of pure C. difficile spores in response to cholate, taurocholate, or glycocholate. Spores were serially diluted and plated onto BHI agar plates containing 0.1% of the indicated germinant. Spores were also enumerated visually with a light microscope. (b) Heat resistance properties of spores. Spores were incubated in distilled water heated to 60°C or 70°C for the indicated times. (c) Chemical resistance properties of spores. Spores were incubated in water, 70% ethanol (EtOH), or 1% Virkon for 20 min at room temperature. After incubation, spores were serially diluted and plated onto BHI agar plates containing 0.5% taurocholate. Germinated spores were counted after 48 h of growth at 37°C under anaerobic conditions. The detection limit of 5 × 10¹ C. difficile CFU/ml is indicated by a dashed horizontal line.

Representation of the C. difficile strain 630 genome, highlighting genes encoding the spore proteome. (a) Circular representation. The concentric circles represent the following (from outside in): first and second circles, all CDS (transcribed clockwise and counterclockwise); third circle, locations of prophage (red), conjugative transposons (blue), and carbohydrate utilization (green) and cell wall (orange) loci; fourth and fifth circles, CDS that code for spore proteins (transcribed clockwise and counterclockwise); sixth circle, G+C content (plotted using a 10-kb window). (b) Linear representation of a 27.8-kb region of the C. difficile strain 630 genome that contains the genes encoding several spore proteins, including a hypothetical cysteine-rich protein (CD1067) that is abundant in C. difficile spores. Red horizontal lines above CDS indicate those that encode proteins found in mature C. difficile spores. The genes are color coded as follows: dark blue, pathogenicity/adaptation; black, energy metabolism; red, information transfer; dark green, surface association; cyan, degradation of large molecules; magenta, degradation of small molecules; yellow, central/intermediary metabolism; pale green, unknown; pale blue, regulators; orange, conserved hypothetical protein; brown, pseudogenes; pink, phage and insertion elements; gray, miscellaneous.