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FEBS Lett. 2009 Jul 21;583(14):2339-42. doi: 10.1016/j.febslet.2009.06.026. Epub 2009 Jun 21.

RNase J is involved in the 5'-end maturation of 16S rRNA and 23S rRNA in Sinorhizobium meliloti.

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  • 1Institut für Mikrobiologie und Molekularbiologie, University of Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.


Sinorhizobium meliloti harbours genes encoding orthologs of ribonuclease (RNase) E and RNase J, the principle endoribonucleases in Escherichia coli and Bacillus subtilis, respectively. To analyse the role of RNase J in S. meliloti, RNA from a mutant with miniTn5-insertion in the RNase J-encoding gene was compared to the wild-type and a difference in the length of the 5.8S-like ribosomal RNA (rRNA) was observed. Complementation of the mutant, Northern blotting and primer extension revealed that RNase J is necessary for the 5'-end maturation of 16S rRNA and of the two 23S rRNA fragments, but not of 5S rRNA.

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