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Biotechniques. 1991 Jul;11(1):14-6.

Chemiluminescent substrates increase sensitivity of antigen detection in western blots.

Author information

  • 1Dept. of Biochemistry and Molecular Biology, Mayo Clinic and Foundation, Rochester, MN 55905.

Abstract

Replacement of colorimetric substrates in Western blots by chemiluminescent reagents enhances the sensitivity of detection by more than an order of magnitude. Protein levels beyond the detection limit of colorimetric substrates can be consistently detected without modifying pre-existing laboratory protocols.

PMID:
1954010
[PubMed - indexed for MEDLINE]
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