Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Eur Biophys J. 2009 Jul;38(6):807-12. doi: 10.1007/s00249-009-0501-6. Epub 2009 Jun 18.

Structured illumination microscopy of a living cell.

Author information

  • 1Randall Division of Cell and Molecular Biophysics, King's College London, New Hunt's House, London, UK.

Abstract

Due to diffraction, the resolution of imaging emitted light in a fluorescence microscope is limited to about 200 nm in the lateral direction. Resolution improvement by a factor of two can be achieved using structured illumination, where a fine grating is projected onto the sample, and the final image is reconstructed from a set of images taken at different grating positions. Here we demonstrate that with the help of a spatial light modulator, this technique can be used for imaging slowly moving structures in living cells.

PMID:
19536533
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Springer
    Loading ...
    Write to the Help Desk