Regulation of the anaphase-promoting complex by the COP9 signalosome

Cell Cycle. 2009 Jul 1;8(13):2041-9. doi: 10.4161/cc.8.13.8850. Epub 2009 Jul 27.

Abstract

The COP9 complex (signalosome) is a known regulator of the proteasome/ubiquitin pathway. Furthermore it regulates the activity of the cullin-RING ligase (CRL) families of ubiquitin E3-complexes. Besides the CRL family, the anaphase-promoting complex (APC/C) is a major regulator of the cell cycle. To investigate a possible connection between both complexes we assessed interacting partners of COP9 using an in vivo protein-protein interaction assay. Hereby, we were able to show for the first time that CSN2, a subunit of the COP9 signalosome, interacts physically with APC/C. Furthermore, we detected a functional influence of the COP9 complex regarding the stability of several targets of the APC/C. Consistent with these data we showed a genetic instability of cells overexpressing CSN2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphase-Promoting Complex-Cyclosome
  • Apoptosis
  • COP9 Signalosome Complex
  • Cell Line, Tumor
  • Genomic Instability
  • Humans
  • Multiprotein Complexes / metabolism*
  • Peptide Hydrolases / metabolism*
  • Repressor Proteins / metabolism*
  • Ubiquitin-Protein Ligase Complexes / metabolism*

Substances

  • COPS2 protein, human
  • Multiprotein Complexes
  • Repressor Proteins
  • Ubiquitin-Protein Ligase Complexes
  • Anaphase-Promoting Complex-Cyclosome
  • Peptide Hydrolases
  • COP9 Signalosome Complex