Permeabilities for monovalent cations, anions, and divalent cations, comparing vector controls (○) and TRa-8 cells (●). (A) Monovalent cations. Permeabilities for monovalent cations resulted in Eisenman sequence IX for the vector control and X for TRa-8 cells, indicating a change to a marginally stronger interaction with unhydrated ions. In general, permeabilities were reduced for all monovalent cations (P_{Li}: n = 4 and 7, *** p < 0.001; P_{K}: n = 6 and 9, *** p < 0.001; P_{Na}: n = 6 and 5, *** p < 0.001; P_{Rb}: n = 5 and 8, *** p < 0.001; P_{Cs}: n = 6 and 15, respectively, *** p < 0.001). (B) Divalent cations. Permeabilities for divalent cations resulted in Sherry sequence III for the mock-transfected and V for TRa-8 cells, showing again a change to a marginally stronger interaction with unhydrated ions. In general, permeabilities were reduced for all divalent cations, except Mg^{2+} (P_{Mg}: n = 9 each; P_{Ca}: 7 each, ** p < 0.01; P_{Sr}: n = 5 each, ** p < 0.01; P_{Ba}: n = 8 and 7, respectively, ** p < 0.01). (C) Monovalent anions. Permeabilities for monovalent anions resulted in Eisenman sequence IV-VII for the vector-transfected cells and III for TRa-8 cells, here suggesting a change to a slightly stronger interaction with hydrated ions. In general, permeabilities were reduced for all measured anions.(P_{Cl}: n = 29 and 28, *** p < 0.001; P_{Br}: n = 10 and 8, *** p < 0.001; P_{I}: n = 5 each, *** p < 0.001).

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