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    Appl Phys Lett. 2008 Oct 13;93(15):153902. Epub 2008 Oct 17.

    Bright-field analysis of phi29 DNA packaging motor using a magnetomechanical system.

    Chang CL, Zhang H, Shu D, Guo P, Savran CA.

    We report a simple and robust magnetomechanical system for direct visual observation of the DNA packaging behavior of the bacteriophage phi29 in real time. The system comprises a micron-sized magnetic bead attached to the free end of the viral DNA, a magnet and a bright-field microscope. We show that the phi29 DNA packaging activity can be observed and dynamically analyzed at the single molecular level in bright field with a relatively simple system. With this system we also visually demonstrate the phi29 motor transporting a cargo 10 000 times the viral size.

    PMID: 19529792 [PubMed]

    PMCID: 2684695

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