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Methods Mol Biol. 2009;548:101-14. doi: 10.1007/978-1-59745-540-4_6.

A genomic approach to yeast chronological aging.

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  • 1Department of Pathology, University of Washington, Seattle, WA 98195-7470, USA.

Abstract

Yeast is a useful model organism to study the genetic and biochemical mechanisms of aging. Genomic studies of aging in yeast have been limited, however, by traditional methodologies that require a large investment of labor and resources. In this chapter, we describe a newly-developed method for quantitatively measuring the chronological life span of each strain contained in the yeast ORF deletion collection. Our approach involves determining population survival by monitoring outgrowth kinetics using a Bioscreen C MBR shaker/incubator/plate reader. This method has accuracy comparable to traditional assays, while allowing for higher throughput and decreased variability in measurement.

PMID:
19521821
[PubMed - indexed for MEDLINE]
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