Graduate School of Frontier Sciences, The University of Tokyo.
Second generation sequencers enabled us to sequence human genome with the speed and the cost 200 to 300 times less than the previous sequencers. Although the short read length is still the problem for reconstitution of copy number variations and genome rearrangements, it is rapidly changing by the development of software and by the improvement of machines. The new sequencers are not only useful for the genome re-sequencing but also for other needs, such as CHIP -seq, DNA modification analysis and transcriptome. We are in the time revolution in the genomics.