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    J Exp Clin Cancer Res. 2009 Jun 5;28:74.

    Upregulation of CENP-H in tongue cancer correlates with poor prognosis and progression.

    Liao WT, Yu CP, Wu DH, Zhang L, Xu LH, Weng GX, Zeng MS, Song LB, Li JS.

    Department of Oral & Maxillofacial Surgery, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, PR China. liaowt2002@gmail.com

    BACKGROUND: Centromere protein H (CENP-H) is one of the fundamental components of the human active kinetochore. Recently, CENP-H was identified to be associated with tumorigenesis. This study was aimed to investigate the clinicopathologic significance of CENP-H in tongue cancer. METHODS: RT-PCR, real time RT-PCR and Western blot were used to examine the expression of CENP-H in tongue cancer cell lines and biopsies. CENP-H protein level in paraffin-embedded tongue cancer tissues were tested by immunohistochemical staining and undergone statistical analysis. CENP-H-knockdown stable cell line was established by infecting cells with a retroviral vector pSuper-retro-CENP-H-siRNA. The biological function of CENP-H was tested by MTT assay, colony formation assay, and Bromodeoxyuridine (BrdU) incorporation assay. RESULTS: CENP-H expression was higher in tongue cancer cell lines and cancer tissues (T) than that in normal cell and adjacent noncancerous tongue tissues (N), respectively. It was overexpressed in 55.95% (94/168) of the paraffin-embedded tongue cancer tissues, and there was a strong correlation between CENP-H expression and clinical stage, as well as T classification. CENP-H can predict the prognosis of tongue cancer patients especially those in early stage. Depletion of CENP-H can inhibit the proliferation of tongue cancer cells (Tca8113) and downregulate the expression of Survivin. CONCLUSION: These findings suggested that CENP-H involves in the development and progression of tongue cancer. CENP-H might be a valuable prognostic indicator for tongue cancer patients within early stage.

    PMID: 19500341 [PubMed - indexed for MEDLINE]

    PMCID: PMC2706220

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