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Opt Express. 2005 Jul 11;13(14):5363-8.

Short-wavelength two-photon excitation fluorescence microscopy of tryptophan with a photonic crystal fiber based light source.

Abstract

We report on a novel and simple light source for short-wavelength two-photon excitation fluorescence microscopy based on the visible nonsolitonic radiation from a photonic crystal fiber. We demonstrate tunability of the light source by varying the wavelength and intensity of the Ti:Sapphire excitation light source. The visible nonsolitonic radiation is used as an excitation light source for two-photon fluorescence microscopy of tryptophan powder.

PMID:
19498530
[PubMed]
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