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Int Arch Allergy Immunol. 2009;149 Suppl 1:21-4. doi: 10.1159/000210649. Epub 2009 Jun 3.

Development of transgenic rice expressing mite antigen for a new concept of immunotherapy.

Author information

  • 1Department of Allergy and Immunology, The Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan.

Abstract

BACKGROUND:

To elucidate the usefulness of antigen-specific immunotherapy based on oral vaccination with an edible part of the plant, we examined the effect of transgenic (Tg) rice seeds expressing an immunodominant fragment of the group 1 antigen of Dermatophagoides pteronyssinus (Der p 1) on a murine model of asthma.

METHODS:

Mice were orally vaccinated with the Tg or non-Tg rice seeds for 2 weeks, then they were immunized with recombinant Der p 1 (rDer p 1) and alum intraperitoneally. Antigen-induced immune responses, such as proliferation and cytokine production of CD4+ T cells, antigen-specific serum IgE and IgG, and infiltration of inflammatory cells into the airways were investigated in those mice.

RESULTS:

The proliferation and Th2 cytokine production of CD4+ T cells in vitro, antigen-specific IgE and IgG synthesis as well as accumulation of eosinophils and lymphocytes into the airways in vivo were significantly inhibited by administration of the Tg rice.

CONCLUSIONS:

These results suggest that the edible vaccines using Tg rice seeds are useful for the treatment of allergic disorders including bronchial asthma.

Copyright 2009 S. Karger AG, Basel.

PMID:
19494500
[PubMed - indexed for MEDLINE]
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