USDA-ARS, Washington State University, Pullman, 99164-6630, USA. cwcunha@vetmed.wsu.edu
A multiplex real-time PCR was developed using a single pair of primers and fluorescent probes specific for five malignant catarrhal fever viruses and an internal positive control. The assay was able to simultaneously detect and differentiate the viruses in clinical samples with high sensitivity (97.2%) and specificity (100%).