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Nat Methods. 2009 Jul;6(7):500-2. Epub 2009 May 31.

Protein interaction platforms: visualization of interacting proteins in yeast.

Schmitz AM, Morrison MF, Agunwamba AO, Nibert ML, Lesser CF.

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Department of Medicine (Microbiology and Molecular Genetics), Division of Infectious Diseases, Massachusetts General Hospital and Harvard Medical School, Cambridge, Massachusetts, USA.

Abstract

Here we describe the protein interaction platform assay, a method for identifying interacting proteins in Saccharomyces cerevisiae. This assay relies on the reovirus scaffolding protein microNS, which forms large focal inclusions in living cells. When a query protein is fused to microNS and potential interaction partners are fused to a fluorescent reporter, interactors can be identified by screening for yeast that display fluorescent foci.

PMID:: 19483691; [PubMed - indexed for MEDLINE]
PMCID: PMC2819136

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