Send to:

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2009;545:243-58. doi: 10.1007/978-1-60327-993-2_15.

The spindle checkpoint: assays for the analysis of spindle checkpoint arrest and recovery.

Author information

  • 1Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK.


The spindle checkpoint is a surveillance mechanism that ensures the fidelity of chromosome segregation by inhibiting anaphase onset until all chromosomes have established stable bipolar attachments. Here we describe a number of protocols that can be used to assay the ability of budding and fission yeast cells to (1) establish and maintain a spindle checkpoint arrest, and (2) segregate chromosomes efficiently upon recovery from mitotic arrest. We focus on experimental detail of the budding yeast protocols, but also point out important differences between budding and fission yeast assays.

[PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Write to the Help Desk