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Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):10059-63.

Primary structure and functional expression of a glutaminyl cyclase.

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  • 1Department of Molecular Neuroendocrinology, Max Planck Institute for Experimental Medicine, Göttingen, Federal Republic of Germany.


A glutaminyl cyclase (QC) that is probably involved in the biosynthesis of pyroglutamyl peptides such as gonadotropin-releasing hormone and thyrotropin-releasing hormone has been purified to homogeneity from bovine anterior pituitary. On the basis of N-terminal sequence analysis, a 2088-base-pair cDNA clone was isolated from a bovine anterior pituitary library. From the nucleotide sequence of this clone, the primary structure of a 330-residue protein and a preceding 31-residue prepropeptide sequence was deduced. By transfection of COS-7 monkey cells with a QC cDNA/pCDM8 vector construct, QC activity was expressed. Hybridization with mRNAs of various bovine tissues revealed expression of QC mainly in brain tissue.

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