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Langmuir. 2009 Dec 15;25(24):13726-9. doi: 10.1021/la901194k.

Oxygen carrier based on hemoglobin/poly(L-lysine)-block-poly(L-phenylalanine) vesicles.

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  • 1State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.

Abstract

An oxygen carrier was prepared by encapsulating carbonylated hemoglobin (CO-Hb) molecules into polypeptide vesicles made from poly(L-lysine)-block-poly(L-phenylalanine) (PLL-b-PPA) diblock copolymers in aqueous medium at pH 5.8. The encapsulation was confirmed by confocal laser scanning microscopy (CLSM). The morphology and size of the vesicles were studied by field-emission scanning electron microscopy (ESEM). They had a spherical shape with a mean diameter of about 4 to 5 microm. The encapsulation efficiency of hemoglobin was 40 wt %, and the hemoglobin content in the vesicles was 32 wt %. The CO-Hb encapsulated in the PLL-b-PPA vesicles was more stable than free CO-Hb under ambient conditions. In the presence of a O(2) atmosphere, the CO-Hb in the vesicle could be converted into oxygen-binding hemoglobin (O(2)-Hb) under irradiation of visible light for 2 h. Therefore, the CO-Hb/PLL-b-PPA vesicles are expected to be used as red blood cell substitutes.

PMID:
19459613
[PubMed - indexed for MEDLINE]
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