Rapid and high-throughput analysis of N-glycans from ovarian cancer serum using a 96-well plate platform

Yun-Gon Kim; Hee-Jin Jeong; Kyoung-Soon Jang; Yung-Hun Yang; Yong-Sang Song; Junho Chung; Byung-Gee Kim

doi:10.1016/j.ab.2009.05.015

Rapid and high-throughput analysis of N-glycans from ovarian cancer serum using a 96-well plate platform

Anal Biochem. 2009 Aug 15;391(2):151-3. doi: 10.1016/j.ab.2009.05.015. Epub 2009 May 18.

Authors

Yun-Gon Kim¹, Hee-Jin Jeong, Kyoung-Soon Jang, Yung-Hun Yang, Yong-Sang Song, Junho Chung, Byung-Gee Kim

Affiliation

¹ Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Republic of Korea.

PMID: 19457428
DOI: 10.1016/j.ab.2009.05.015

Abstract

We present a rapid and high-throughput human serum N-glycan preparation technology using 96-well plate-based procedures. The released N-glycans from polyvinylidene fluoride (PVDF) membrane filter plate are subsequently loaded to porous graphitic carbon (PGC) containing a 96-well plate to remove salts and other contaminants without sacrificing accuracy or reproducibility. This robust glycan preparation technology is applied to ovarian cancer diagnosis using 5 microl of patient serum.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Female
Humans
Membranes, Artificial
Ovarian Neoplasms / blood
Ovarian Neoplasms / chemistry*
Polysaccharides / blood*
Polysaccharides / isolation & purification
Polyvinyls / chemistry
Solid Phase Extraction / instrumentation*
Solid Phase Extraction / methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Membranes, Artificial
Polysaccharides
Polyvinyls
polyvinylidene fluoride