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Mamm Genome. 2009 Jun;20(6):327-38. doi: 10.1007/s00335-009-9187-4. Epub 2009 May 19.

Next-generation sequencing of vertebrate experimental organisms.

Author information

  • 1Experimental Cancer Genetics, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK.

Abstract

Next-generation sequencing technologies are revolutionizing biology by allowing for genome-wide transcription factor binding-site profiling, transcriptome sequencing, and more recently, whole-genome resequencing. While it is currently not possible to generate complete de novo assemblies of higher-vertebrate genomes using next-generation sequencing, improvements in sequence read lengths and throughput, coupled with new assembly algorithms for large data sets, will soon make this a reality. These developments will in turn spawn a revolution in how genomic data are used to understand genetics and how model organisms are used for disease gene discovery. This review provides an overview of the current next-generation sequencing platforms and the newest computational tools for the analysis of next-generation sequencing data. We also describe how next-generation sequencing may be applied in the context of vertebrate model organism genetics.

PMID:
19452216
[PubMed - indexed for MEDLINE]
PMCID:
PMC2714443
Free PMC Article

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