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Biophys J. 2009 May 20;96(10):4268-75. doi: 10.1016/j.bpj.2009.02.045.

Velocity, processivity, and individual steps of single myosin V molecules in live cells.

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  • 1Institut Curie, Centre de Recherche, Laboratoire Physico-Chimie Curie, Centre National de la Recherche Scientifique, UMR 168, Université Pierre et Marie Curie-Paris, Paris, France.

Abstract

We report the tracking of single myosin V molecules in their natural environment, the cell. Myosin V molecules, labeled with quantum dots, are introduced into the cytoplasm of living HeLa cells and their motion is recorded at the single molecule level with high spatial and temporal resolution. We perform an intracellular measurement of key parameters of this molecular transporter: velocity, processivity, step size, and dwell time. Our experiments bridge the gap between in vitro single molecule assays and the indirect measurements of the motor features deduced from the tracking of organelles in live cells.

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PMID:
19450497
[PubMed - indexed for MEDLINE]
PMCID:
PMC2712235
Free PMC Article

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