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Vaccine. 2009 Jun 12;27(29):3862-9. doi: 10.1016/j.vaccine.2009.04.019. Epub 2009 May 3.

H5N1 strain-specific hemagglutinin CD4+ T cell epitopes restricted by HLA DR4.

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  • 1Benaroya Research Institute at Virginia Mason, Seattle, WA 98101-2795, United States.

Abstract

CD4+ T cells play a pivotal role in the viral immunity, and as such identification of unique strain-specific HLA class II restricted epitopes is essential for monitoring cellular strain-specific viral immunity. Using Tetramer-Guided Epitope Mapping technique, we identified HLA-DR0401 restricted HA epitopes that are strain-specific to H5N1 virion. Two immunodominant epitopes H5HA(441-460) and H5HA(57-76) were identified from in vitro stimulated human PBMC. Both epitopes elicit strong cellular immune responses when HLA-DR0401 transgenic mice are immunized with H5N1 subvirion indicating in vivo naturally processed immunodominant epitopes. The H5HA(57-76) epitope is unique for the H5N1 strain but conserved among all H5N1 clades recommended for vaccine development by World Health Organization. The unique H5HA(57-76) response was uncommon in unexposed individuals and only observed in the naïve T cell subset. Thus, H5N1 strain-specific H5HA(57-76) immunogenic epitope represents a unique marker for monitoring the efficacy of vaccination or as a candidate vaccine peptide.

PMID:
19446935
[PubMed - indexed for MEDLINE]
PMCID:
PMC2695938
Free PMC Article

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