(A) Immunochemical analyses on successive frozen rat pancreas sections stained for insulin (brown) and PSA-NCAM (purple). ex: exocrine tissue ; en: endocrine tissue. (B) Frozen rat pancreas. In islets, PSA-NCAM (red) colocalizes only with insulin (green) and not with other islet hormones (blue). Scale bar: 30 µm. (C) Dissociated islet cells. PSA-NCAM (red) colocalizes only with insulin (green) and not with other islet hormones (blue). Scale bar: 10 µm. (D) Representative dot plot analysis of dissociated islet cells examined for their FAD content and cell size (FSC) in order to gate total β-cells (black frame). (E) Representative histogram of PSA-NCAM surface labeling of total β-cells. Vertical arrows indicate the geometric mean (481±27 for labeled β-cells). This value was used to arbitrarily separate and sort highly (β^high) and poorly (β^low) PSA-NCAM labeled β-cells (n = 7). (F) Immunoblot analysis for total PSA-NCAM expression. Cyclophilin was used as loading control.

(A) Insulin release (top panel) and cytoplasmic calcium concentration ([Ca²⁺]i) oscillations (bottom panel) in response to 5.5 mM and 16.7 mM glucose in β^high and β^low-cells during perifusion experiments (n = 3–6). Quantification of insulin and calcium responses to stimulating glucose are represented by ΔI and ΔR respectively. (B) Insulin release (top panel) and intracellular ATP ([ATP]) levels (bottom panel) in response to 5.5 mM and 16.7 mM glucose in β^high and β^low cells assessed by static incubation experiments (n = 2). n represents the number of independent cell preparations from 6 pooled rats each. Data are means±SEM. ***, p<0.005.

(A) Effects of 5.5 mM and 16.7 mM glucose±10 mM leucine (Leu) or 19 mM arginine (Arg) on insulin release in β^high and β^low-cells (n = 3–5). Data are means±SEM. *, p<0.05 and **, p<0.01 for stimulating conditions (16.7 mM glucose±Arg or Leu) compared to basal conditions (5.5 mM glucose±Arg or Leu). ¤, p<0.05 and ¤¤, p<0.01 between treated (glucose+Arg or Leu) and non-treated conditions (glucose alone) for same glucose concentrations. ££, p<0.01 for Arg or Leu alone compared to 5.5 mM glucose alone. (B) Insulin secretory response to 5.5 mM glucose±50 mM KCl in β^high and β^low-cells (n = 3–5). Quantification of insulin response to KCl is represented by ΔI. Data are means±SEM. *, p<0.05 between β^high and β^low-cells. n represents the number of independent cell preparations from 6 pooled rats each.

(A) Insulinogenic index (ΔI/ΔG) after OGTT in ZDF fa/fa rats compared to ZDF lean controls (12 rats). (B) Representative FACS analyses of dissociated islet cells in type 2 diabetic ZDF rat, a model of loss of β-cell function. The geometric mean of PSA-NCAM fluorescence is represented by a blue arrow in control ZDF lean rats and by a red arrow in diabetic ZDF fa/fa rats. The distribution of β^high and β^low-cells is determined by the geometric mean of PSA-NCAM of the control ZDF lean rats (blue arrows) and expressed in percent of total β-cells (n = 3–6). (C) Insulin release in response to 5.5 mM and 16.7 mM glucose in β^high and β^low-cells of ZDF rats during perifusion experiments (n = 3–6). Quantification of insulin response to stimulating glucose is represented by ΔI. (D) Insulinogenic index (ΔI/ΔG) after OGTT in HG/HI rats compared to saline-infused controls (Wistar control). (E) Representative FACS analyses of dissociated islet cells in HG/HI rats and Wistar control rats. The geometric mean of PSA-NCAM fluorescence is represented by a blue arrow in saline-infused Wistar control rats and by a red arrow in HG/HI rats. The distribution of β^high and β^low-cells is determined by the geometric mean of PSA-NCAM of the control Wistar rats (blue arrows) and expressed in percent of total β-cells (n = 3–6). (F) Insulin release in response to 5.5 mM and 16.7 mM glucose in β^high and β^low-cells of control and HG/HI rats during perifusion experiments (n = 3–6). Quantification of insulin response to stimulating glucose is represented by ΔI. n represents the number of independent cell preparations from at least 6 rats pooled each. Data are means±SEM. *, p<0.05; ***, p<0.005.

(A) Insulin release in response to 5.5 mM, 8.3 mM and 16.7 mM glucose in β^high and β^low cells determined by perifusion experiments (n = 2). Quantification of insulin response to stimulating glucose is represented by ΔI. (B) Insulin content (n = 3). (C–E) Representative histogram of size (FSC) (C), FAD (autofluorescence) (D) and granularity (SSC) (E) of sorted β^high and β^low-cells (n = 5–7). (F) Immunoblot analysis for PSA-NCAM expression after endoneuraminidase N (endoN) treatment (0.7 U/ml) of β^high-cells. Cyclophilin was used as loading control. (G) Insulin release in response to 5.5 mM and 16.7 mM glucose in endoN-treated β^high-cells (0.7 U/ml) assessed by static incubation experiments (n = 2). n represents the number of independent cell preparations from 6 pooled rats each. Data are means±SEM. *, p<0.05; **, p<0.01; ***, p<0.005.

Effects of 5.5 mM and 16.7 mM glucose±10 nM GLP-1 on insulin release (A) and cAMP content (B) in β^high and β^low-cells assessed by static incubation experiments (n = 3–5). (C) Effects of 5.5 mM and 16.7 mM glucose±5 mM db-cAMP on insulin secretion in β^high and β^low-cells (n = 3–5). n represents the number of independent cell preparations from 6 pooled rats each. Data are means±SEM. *, p<0.05 and **, p<0.01.

(A) Representative pictures of actin filament distribution in β^high and β^low-cells. Scale bar: 10 µm (B) Representative immunoblots of exocytotic proteins in β^high and β^low-cells (n = 3). n represents the number of independent cell preparations from at least 6 pooled rats each.