Format

Send to:

Choose Destination
See comment in PubMed Commons below
Free Radic Biol Med. 2009 Apr 15;46(8):965-88. doi: 10.1016/j.freeradbiomed.2009.01.007. Epub 2009 Jan 21.

Quantification of protein modification by oxidants.

Author information

  • 1The Heart Research Institute, Camperdown, NSW, Australia.

Abstract

Proteins are major targets for oxidative damage due to their abundance and rapid rates of reaction with a wide range of radicals and excited state species, such as singlet oxygen. Exposure of proteins to these oxidants results in loss of the parent amino acid residue, formation of unstable intermediates, and the generation of stable products. Each of these events can be used, to a greater or lesser extent, to quantify damage to proteins. In this review the advantages and disadvantages of a number of these approaches are discussed, with an emphasis on methods that yield absolute quantitative data on the extent of protein modification. Detailed methods sheets are provided for many of these techniques.

PMID:
19439229
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk