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J Nanosci Nanotechnol. 2009 Apr;9(4):2566-73.

Synthesis, properties, and surface enhanced Raman scattering of gold and silver nanoparticles in chitosan matrix.

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  • 1State Key Laboratory of Bioelectronics, Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China.


We present here a facile route to in-situsynthesis of free-standing metal (gold or silver) nanoparticles-embedded chitosan films by thermal treatment. The produced nanoparticles were confirmed by UV-vis spectroscopy and transmission electron microscopy (TEM). Interestingly, an exquisite dendritic structure was observed in the resulting silver-chitosan film, which was not present in pure chitosan and gold-chitosan samples. We speculated that the formation of dendritic structures may be due to the presence of nano-sized silver particles which has an influence on the crystallization behavior and thereby the morphology of biopolyer chitosan. The application of the as-prepared metal-chitosan films in surface-enhanced Raman spectroscopy (SERS) was investigated by using Rhodamine 6G (R6G) as probe molecules. It was found that the resultant metal-chitosan samples, especially silver-chitosan one, could be used as SERS substrates exhibiting excellent enhancement ability.

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