Preliminary investigation of crystals of the neutral lipase from Pseudomonas fluorescens

J Mol Biol. 1991 Nov 5;222(1):21-2. doi: 10.1016/0022-2836(91)90732-l.

Abstract

The neutral lipase from the bacteria Pseudomonas fluorescens, marketed under the trade name LpL-200S, has been crystallized in a form suitable for X-ray diffraction analysis from 35% n-propanol at pH 8.5. The crystals are monoclinic prisms and are of space group C2 with a = 91.00 A, b = 47.17 A, c = 35.21 A and beta = 121.43 degrees. There is one molecule of the protein as the asymmetric unit of the crystals. The diffraction pattern extends to at least 1.6 A resolution and the crystals are extremely robust in terms of X-ray exposure.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Crystallization
  • Lipase / ultrastructure*
  • Pseudomonas fluorescens / enzymology*
  • Pseudomonas fluorescens / ultrastructure
  • X-Ray Diffraction

Substances

  • Lipase