Chembiochem. 2009 May 25;10(8):1297-301.

Functional analysis of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin macrolide antibiotics.

Li S, Anzai Y, Kinoshita K, Kato F, Sherman DH.

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Life Sciences Institute, Department of Medicinal Chemistry, Chemistry, and Microbiology and Immunology, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI 48109-2216, USA.

Abstract

Mg motors: We characterized the in vitro function of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin antibiotics. Each enzyme was confirmed to be an S-adenosyl-L-methionine (SAM)-dependent deoxysugar methyltransferase. Their optimal activities require the presence of Mg(2+). With the reconstituted in vitro assays, the order of mycinamicin VI-->III-->IV in the post-PKS (polyketide synthase) tailoring pathway of mycinamicin was unambiguously determined.

PMID:: 19415708; [PubMed - indexed for MEDLINE]
PMCID:: PMC2861363

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